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Product Info Summary
| SKU: | PB9380 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospholipase D1/PLD1 Antibody Picoband™
SKU/Catalog Number
PB9380
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Phospholipase D1/PLD1 Antibody Picoband™ catalog # PB9380. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospholipase D1/PLD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9380)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PLD1 recombinant protein (Position: M1-H330). Human PLD1 shares 91.8% and 90.9% amino acid (aa) sequence identity with mouse and rat PLD1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9380 is reactive to PLD1 in Human, Mouse
Applications
PB9380 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
124 kDa
Calculated molecular weight
124.184kDa
Background of PLD1
By somatic cell hybrid analysis, PLD1 is mapped to 3q26.31. This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. And PLD1 disrupted association of gamma-secretase protein components, independent of PLD1catalytic activity. What's more, PLD1 regulates intracellular trafficking of beta-amyloid, distinct from its effect on gamma-secretase activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-PLD1 Picoband antibody, PB9380, Western blotting
All lanes: Anti PLD1 (PB9380) at 0.5ug/ml
Lane 1: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 124KD
Observed bind size: 124KD
Protein Target Info & Infographic
Gene/Protein Information For PLD1 (Source: Uniprot.org, NCBI)
Gene Name
PLD1
Full Name
Phospholipase D1
Weight
124.184kDa
Superfamily
phospholipase D family
Alternative Names
Choline phosphatase 1; EC 3.1.4.4; hPLD1; Phosphatidylcholine-hydrolyzing phospholipase D1; phospholipase D1; phospholipase D1, phophatidylcholine-specific; phospholipase D1, phosphatidylcholine-specific; PLD 1; PLD1 PLD1 CVDD phospholipase D1 phospholipase D1|choline phosphatase 1|phosphatidylcholine-hydrolyzing phospholipase D1|phospholipase D1, phosphatidylcholine-specific
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLD1, check out the PLD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospholipase D1/PLD1 Antibody Picoband™ (PB9380)
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No publications found for PB9380
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-Phospholipase D1/PLD1 Antibody Picoband™
Question
I was wanting to use your anti-Phospholipase D1/PLD1 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
You can see on the product datasheet, PB9380 anti-Phospholipase D1/PLD1 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-Phospholipase D1/PLD1 antibody PB9380 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The anti-Phospholipase D1/PLD1 antibody (PB9380) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-22
Question
See below the WB image, lot number and protocol we used for brain using anti-Phospholipase D1/PLD1 antibody PB9380. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-11
